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Therefore, while there are certainly limitations that need to be considered when a reactive metabolite is added exogenously to a cell rather than generated intracellularly, for certain reactive metabolites the profiles of modification outside and inside a cell may be similar.
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To accomplish this, we measured the profiles of H3K4me3 modification along the length of three groups of genes: all expressed genes, dehydration-induced genes, and the subset of dehydration induced genes that were also ABA induced [ 21].
To confirm the distribution profiles of histone modifications, we calculated the ratio of histone modifications (H3K4me3 and H3K9ac) to pan-H3.
Next, we examined the distribution profiles of histone modifications and during different stages of P. falciparum IEC and compared it with profiles of histone modifications in human (Additional file 1: Table S3).
To date, the profiles of histone modifications during stem cell differentiation process, which may be tightly associated with the gene expression patterns, have not been extensively studied.
Our data establish ING2 as a chromatin-associated, non-enzyme protein that is critical to temporal and spatial profiles of chromatin modifications during spermatogenesis.
Average profiles of histone modifications on MLL4+ MyoD+ active enhancers are shown.
Average profiles of histone modifications, MED1 and Pol II on MLL4+ adipogenic enhancers are shown.
The profiles of histone modifications H3K36me2 and H3K36me3 in P. falciparum, which are associated with transcription elongation in human, match closely with that of humans profile (Fig. 2).
We then investigate profiles of histone modifications by ChIP-Seq, for H3 Lysine 4 (H3K4me) and acetylation of H3 Lysine 27 (H3K27ac), in the presence or absence of acupuncture treatment.
Genomic loci that are associated with similar enrichment profiles of histone modifications regardless of cell type were specifically chosen for this analysis.
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