The expression data were median and log normalized to yield relative tissue-specific gene expression profiles, and these profiles were separated into 20 co-expressed groups of genes using k-means clustering.
Profiles were separated into DAPI, 1n (1c) and 2n (2c) peaks.
Typical bands from the DGGE profiles were separated, re-amplified, sequenced and thirteen sequences were obtained.
Equal amounts of protein were loaded for each sample and the extracted protein profiles were separated by SDS-PAGE.
Principle component analysis (PCA) showed that the transcription profiles were separated according to genotype on PC1, which explained 66%% of the variance (Fig. 5).
Based on previous preliminary studies, drug release profiles were separated into four phases, namely, the initial phase, the first pulse of drug release, the lag phase, and second phase of drug release.
cDNA-AFLP profiles were separated on Li-Cor IR 4200S automated DNA sequencers using an 8% (v/v) LongRanger acrylamide gel solution (Cambrex Corp., East Rutherford NJ, USA) as previously described (Myburg et al., 2001).
Because the p Ka values of the acidic and basic limbs of the PTDH pH rate profile are separated by <2 pH units, it is difficult to differentiate between normal and reverse protonation states.
The cross pattern is rotated by 26° with respect to the flight direction, to optimize the coverage of the surface with the five profiles: each profile is separated by about 10 m cross-track, and the average along-track distance between spots is also 10 m.
