Exact(3)
The GPCR Profiler screen uses real-time calcium flux FLIPR assay.
Preliminary hits from the profiler screen (<40% kinase activity remaining) were validated as true positives or false hits by a follow-up determination of an IC50 value.
The final concentration of MW150 in the profiler screen (20 000 nM) was achieved by serial dilution of a DMSO stock solution to the final assay concentration in 2.0% DMSO/Tris buffer.
Similar(56)
All respondents went through a health care profiler (screening questions) to ensure that their diabetes had been diagnosed by a physician and that a relevant treatment was initiated.
To analyse the molecular basis of this angiogenic activation of OECs in the coculture system, a human angiogenesis RT² profiler array (PCR array) screening the expression of more than 80 genes involved in the process of angiogenesis was performed after one and four weeks of cocultivation.
See Profile: Statistical Profiler for more information.
We used the Epithelial to Mesenchymal Transition RT Profiler PCR Arrays (Qiagen) to screen the mRNA expression of multiple genes related to EMT in HCT116 cells, which were transfected with the specific miR-200a inhibitor and mimetic in comparison with the respective control (miScript negative Control, Qiagen).
Some of the gene screened by PCR RT2 Profiler™ APM-013-2 APM-013-2 APM-013-2feresultede profine expression when comparedifferenttrigene
To further understand the molecular basis underlying FMOD's wound healing enhancing effects, a RT2 profiler PCR array was utilized to screen for FMOD-responsive genes during the adult rat wound healing period (Supplementary Figure 5).
The screening results from the RT² Profiler Apoptosis PCR Array showed that SF attenuated the gene expressions of TNFR-1, TRADD, caspase-8 and caspase-3, which were related to the TNF/TNFR-induced caspase cascade apoptosis pathway.
Increasingly, this may mean the creation of a space of gauntlets and gates, where passengers are screened by profilers, sniffed by dogs, zapped by magnetometers and analyzed by spectrographs for any untoward residue.
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