Before the measurements, the profiler was calibrated under the treatment condition.
We recorded water conditions using an Acoustic Doppler Current Profiler.
These profiles were measured with a Profiler.
To determine which angiogenic factors are produced by RA FLS in response to hypoxia, we isolated RA FLS from synovial tissue of five patients, cultured them for 4 or 24 hours under either hypoxic conditions (1% oxygen) or normoxic conditions (21% oxygen), and screened the cDNA using a Human Angiogenesis RT2 Profiler™ PCR array.
The most accurate discharge estimation methods are the weir, the acoustic Doppler current profiler, and the in-stream velocity area method; however, under certain conditions each of these methods is less desirable than other methods.
Primer sequences are shown in Online Resource 1. Secreted cytokine expression was assessed in pooled conditioned media from each experimental group using a Proteome Profiler™ antibody array (R&D Systems).
In addition, all determined transcription levels for each gene at each temperature condition were analyzed by the use of the T-profiler, a tool that uses the t-test to score changes in the average activity of predefined groups of genes [ 16, 17].
A 3D optical profiler was used (Wyko NT 1100, Veeco, Santa Barbara, CA, USA) under the following conditions: vertical scanning mode, Mirau lens, ×20.3 magnification (231.1 × 303.8 μm2 analysis area), tilt correction, 5-μm Gaussian high-pass filter, and 0.2 μm (x, y) and 0.1 nm (z) resolution.
We have come to the conclusion that the use of the Chapman profiler should be exercised with precaution as there are evidences that there are conditions when other profilers are better fit for modelling purposes.
Simulation profiler is then generated to verify its insensitivity to the variations in the operating conditions and the inferences on relationships between power density and operating parameters are investigated.
