To determine if similar epigenetic modifications are induced by long-term culture of fibroblasts, we have compared DNA methylation profiles of passage 3 and passage 21 of fibroblasts (6 year old donor, othoplastic).
We built a comprehensive gene association network to model angiogenesis regulation via complementary profiling of passage dependent gene expression in two cell types that constituted the co-culture model.
To investigate the molecular mechanism behind the cellular senescence of hBMMSCs, microarray analysis was used to compare the expression profiles of early passage hBMMSCs, late passage hBMMSCs and hBMMSCs ectopically expressing human telomerase reverse transcriptase (hTERT).
DNA methylation profiles of early passage (P5) and later passage (P10) of the four samples were compared using the Human-Methylation27 BeadChip microarray.
Gene expression profiling of early passage, presenescent TCCs compared to late passage, senescent clones demonstrated that 43 genes were differentially expressed in T-cell immunosenescence.
To address whether gene expression in breast cancer associated fibroblasts varies between breast cancer subtypes, we compared the gene expression profiles of early passage primary CAFs isolated from twenty human breast cancer samples representing three main subtypes; seven ER+, seven triple negative (TNBC) and six Her2+.
The Toronto Star has reported extensively over the past few years on the problem of racial profiling in Canada's most populous city, calling being profiled a "rite of passage for many black men".
In Figure 3, the lesion profile of 139A after passage in fish was a mean of 4 animals when brains for histology were available (2 mice inoculated with turbot spleen at 15 days, 1 mouse inoculated with trout spleen at 15 days, and 1 turbot brain taken 90 days after parenteral inoculation).
To examine the stability of DNA RT profiles of serially passaged xenografts of primary human B- and T-ALL cells, we first devised a method that circumvents the need for bromodeoxyuridine incorporation to distinguish early versus late S-phase cells.
Some genes (OTX2, RAX, CDKN2B, COL1A1, and COL1A2) even have apparent cyclical expression profiles as a function of passage.
