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Second, the profile intensity of the staining along each line was plotted.
For the statistical analysis of IF images, four different random images from each condition were analyzed using the profile intensity function of the Zen confocal software (Zeiss).
Then, for each central gene of windows of size 2 r + 1, the term/pathway profile intensity is defined as the average over the window.
To outline the proximity of the fluorescently-marked Caveolin-1 and β-catenin proteins, profile intensity plots were generated in LAS-AF imaging software (Leica microsystems, Wetzlar, Germany).
The effect of changing the pump laser wavelength from on resonance to an off resonance frequency clearly produces a change in profile intensity.
Profile intensity plots showed clear signal peaks of β-catenin expression within the cell nucleus of NCs, indicative of active canonical Wnt signaling.
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Complete information on wind (mean velocity profile, turbulence intensity, power spectrum of the fluctuation, etc).
The orthogonal frame at the focus location provided a time profile of intensity levels.
Association between the asymptomatic profile and intensity of antigen recognition was verified by logistic regression.
Secondary endpoints included safety profile, dose intensity (mg m−2 per week), relapse-free survival and overall survival.
We then calculated the negative first derivative of the plot of the resulting melting profile of intensity versus temperature.
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