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Both products were subsequently reduced to obtain the free thiols.
These second round PCR products were subsequently sequenced.
Ligation products were subsequently cleaned up using 1x AMPure XP beads.
The PCR products were subsequently subjected to direct sequencing PCR with BigDye terminator V 3.0 cycle sequencing reagents (Applied Biosystems).
Cloned PCR products were subsequently screened by amplified rDNA restriction analysis to identify operational taxonomic units (OTUs).
The PCR products were subsequently subcloned for sequencing.
Products were subsequently run on 4% agarose gels for analysis.
The PCR products were subsequently purified and sequenced in two directions on an ABI 3700 automated sequencer.
The PCR products were subsequently separated in 6.0 % polyacrylamide gel and visualized using the silver-staining approach (Bassam et al. 1991).
PCR products were subsequently separated on a SSCP gel.
Amplified PCR products were subsequently cleaned by the Exo-SAP method [17].
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