The products were further characterized using TEM (Figs. 2d f).
Positive PCR-amplified products were further sequenced, and a phylogenetic tree was constructed by neighbor joining of the V4 and V5 sequences from 15 prototypes strains.
The reaction products were further characterized by UV visible spectroscopy.
The products were further analyzed using grazing-incident X-ray diffraction (XRD).
The amplified products were further checked by electrophoresis in 1 % agarose gel in 1X TBE buffer.
The products were further characterized by HRTEM, shown in Figure 3d.
The PCR products were further verified, purified, and sequenced as mentioned above.
The resultant solid products were further purified by dialysis and ultrafiltration for cell imaging.
The structure of the bis-chalcone and its cyclized products were further confirmed by the corresponding 1H-NMR spectra.
Amplified products were further analyzed by the technique of denaturing gradient gel electrophoresis to confirm that they were successfully isolated.
The chemical compositions of solid products were further characterized using FTIR spectroscopy as shown in Fig. 3a.
