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Meat products samples were collected from street sellers as braised meat.
For ubiquitinated products, samples were separated on a 10% and 8% (bottom and top, respectively) denaturing gel prior to transfer.
Following ethanol precipitation of the sequenced products, samples were analysed using an ABI 3730 DNA sequencer (Perkin Elmer).
In order to determine the concentrations of fermentation products, samples taken from the fermentation flasks were centrifuged and the supernatants were filtered through 0.2 μm filters.
For detection and quantification of Gln/Gln and their enzymatic reaction products, samples were labeled with a series of TMT reagents (Thermo, Pierce Biotechnology, Rockford, IL, USA) according to the manufacturer's instruction.
For analysis of the oxidized products, samples treated by laccase and mediators and by laccase and mediators and NaClO2 oxidation were enzymatically hydrolyzed into soluble sugars with Celluclast 1.5L (40 mg g−1 DM of substrate) and Novozym 188 (1,000 nkat g−1 DM).
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The agency said "residues found in agricultural products sampled are at levels that do not pose risk to consumers' health and are safe..."...
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