Sentence examples for products lengths from inspiring English sources

The difference of PCR products lengths between strains of two mating types (marked as KO-1 and KO-2) was reasonable for a deleted region larger than target sequence, and it was often observed during the process of co-deletion (Hayashi and Mochizuki, 2015).

Because the PCR products span two introns, most of the products' lengths would exceed 400 bp if using the genomic DNA as template.

PCR products (lengths 3665 and 3574 bp for NUS and mNUS, respectively) were cloned into pGL3 basic vector (Promega) upstream the reporter gene for Luciferase using MluI and BglII restriction sites.

We also PCR-amplified seven exon-exon junctions of ENSTGUG00000017495, and in all cases obtained products lengths consistent with the presence of introns and of similar size as expected from intron length in zebra finch (R2 = 0.93, p < 0.001).

Primers, amplification protocols and PCR products lengths for these loci are represented in Additional file 2. In all cases, PCR mixtures were prepared under similar conditions in a final volume of 25 μl containing 1-2 μl DNA, 0.5 μM each primer, 0.2 mM each dNTP, 1.5 mM MgCl2, and 1 unit of Taq DNA polymerase (Invitrogen).

The PCR amplification utilized 3 min at 94°C followed by 25 cycles consisting of 94°C for 1 min, 55°C for 1 min and 72°C for 2 min with a final extension at 72°C for 10 min. The PCR products (lengths ranging from 900 to 1100 bp) were ligated into the pCRII-TOPO vector (Invitrogen) and subsequently used for the transformation of E. coli-XL1-blue cells (Stratagene).

Primers were chosen to be 20 bp in length, with a GC content of 45 60%, no long repeats of a single base, and 150 500 bp of products length.

Primer sequences, annealing temperatures and PCR products length for all the TLRs analyzed, as well as for β-actin are shown in Table 1.

Identifiers and product lengths of reference genes primers used in this study.

The presence of leptin receptor isoforms in prostate cancer cells was assessed using RT-PCR and expected product lengths were verified by gel electrophoresis.

PCR primers were designed to give product lengths ranging from 67 to 127 nucleotides (Table S1).