Additionally, demands for increased productivity, wider substrate range utilization, and production of nonconventional compounds in industry, as well as changing consumer preferences, lead to a great interest in further improving the currently used industrial strains and the selection or development of strains with novel properties.
The parameters of the feed cyclic functions are determined which resulted in improved productivity and substrate consumption.
Additionally, computational methods have been developed to suggest different types of genetic modifications (e.g. gene deletion/addition or up/down regulation), as well as suggest strategies meeting different criteria (e.g. high yield, high productivity, or substrate co-utilization).
The engineering of cells for the production of fuels and chemicals involves simultaneous optimization of multiple objectives, such as specific productivity, extended substrate range and improved tolerance – all under a great degree of uncertainty.
Compared with pure culture of single strain, mixed culture of microbial consortium normally showed higher efficiency or productivity and substrate tolerance.
Spatial variation in two prey defense traits, crushing resistance and shell pigmentation, exhibits striking variation at small spatial scales often associated with habitat differences in primary productivity and substrate coloration.
Cultivation in the medium containing 20 g/L NaCl, with optimized feeding of glycerol and ammonia, resulted in a PHB content of 66.9% of cell dry weight, and the polymer productivity and substrate yield coefficient of 1.09 g/L/h and 0.25 g PHB/g glycerol, respectively [ 35].
Considering the volumetric productivities and substrate tolerances of the above mentioned bacteria, C. butyricum is amongst the best producers (Wilkens et al. [2012]).
C. saccharolyticus with its versatile H2-production activity augments biogas productivity from various substrates to a similar extent to E. cloacae.
We further analyze how sensitive the productivity is to substrate uptake and growth parameters.
