Whilst the future does looks bright for this sector, the emergence of biosimilar competition is ensuring that manufacturers of these products are continuously seeking methods of increasing productivity of cell culture processes to maximise yields.
Moreover, declined glucose concentration after ultrasonication from 1.16 to 0.46 mg/l after 7 days of incubation, implies that cellular uptake of substrate was increased and productivity of cell was also enhanced.
Hence the productivity of cell cultures has improved more than 100-fold in the last two decades mainly due to developments of fed-batch culture systems, media and process optimisations in conjunction with expression technologies [ 1, 2].
The relative IL-10 productivity of B1 cells and MZ B cell populations in vivo is not clear.
Typical cell line engineering strategy focuses on improving time integral of viable cell density (IVCD) and specific protein productivity (qp) of cells [ 11- 16].
By enriching with the 10% strongest DsRed-fluorescent cells, the productivity of both cell populations could be successfully improved.
To the best of our knowledge, this is the first time that an engineered decrease in the number of silent rRNA genes could be correlated with enhanced production of rRNA and ribosomes and consequently with higher productivity of mammalian cells.
