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Protein production was validated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using a 5 20% e-PAGEL (ATTO, Tokyo, Japan) and Full Range RPN800E protein markers (GE healthcare, Little Chalfont, UK).
The effect of each gene on melanin production was validated by the use of knockout mutants.
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Moreover, new gene amplification targets for further enhancing putrescine production were validated through experiments involving the overexpression of each identified targeted gene under condition-controlled batch cultivation.
The applicability of the framework for a full scale lignite production environment was validated and significant improvements were demonstrated.
Following the lysis procedure, FliC secretion and production of JOL1485 was validated by immunoblot analysis.
The novel feature of erythrocytes production by PGCCs was validated in different experiments.
In this research, the process of biodiesel production from beef tallow was validated, but the economical viability must be improved by recovering methanol and glycerol.
The simulation of soil moisture dynamics and vegetation production by the model was validated by comparison with independent data that were not used to parameterize the model (for details see Additional file 7: Validation.pdf).
ZapSib's current managers said they reinvested profits to expand production, a decision they said was validated by the mill's return to profit after years of steady losses.
The accuracy of simply weighing daily faecal production to identify gastrointestinal dysfunction was validated against three reference methods [ 1].
This result suggested that our hypothesis regarding the increase in l-glutamic acid production by pgk gene amplification was validated qualitatively.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com