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Enzyme production was quantified using the Morgan-Elson assay that detects the breakdown products of CSPG digestion in cell supernatants.
Viral DNA production was quantified using a novel real time PCR assay.
Intracellular and extracellular nitrite production was quantified using the Griess reagent kit (Molecular Probes) following the manufacturer's instructions.
ROS production was quantified using a fluorescent plate reader (VICTOR X2, PerkinElmer) with 485-nm emission and 525-nm absorption.
vDNA production was quantified using the 5NCR forward primer 5'GGCTAACTAGGGAACCCACTGCTT-3', the 5NCR reverse primer 5'-AGCCGAGTCCTGCGTCG-3', and the 5NCR probe 5'- FAM -CCTCAATAAAGCTTGCCTTGAGTGCTTCAA- TAMRA -3'- FAM -CCTCAATAAAGCTTGCCTTGAGTGCTTCAA- TAMRA -3
Hydrogen production was quantified using DTH-reduced methyl viologen (5 mM) or the ferredoxin PetF from Synechocystis (50 µM) as the electron donating substrate.
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ALP activity, Coll I production, and OC production were quantified using the Rabbit Total Alkaline Phosphatase (TALP) enzyme-linked immunosorbent assay (ELISA) Kit, the Rabbit Collagen Type I, Col I ELISA Kit, and the Rabbit Osteocalcin/bone gla protein ELISA Kit (all from Sino-American Biotechnology, Wuhan, China), respectively, according to the manufacturer's instructions.
Mass loss was measured after each 50 K step and the production of pyrolysis products was quantified using gas chromatography techniques.
PCR products were quantified using ImageJ.
The collagen production on scaffolds was quantified using Sircol collagen dye binding assay Kit (Biocolor Ltd., Newtown, Ireland).
The production of Stx2 was quantified using polymixin lysis as described in Ziebell et al. [ 34], with some modification.
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