Exact(4)
Cells were treated with AAP at different concentrations for 18 hours and then the rate of ROS production was monitored using the fluorescent dye CM-H2-DCFDA.
To test the hypothesis that DETC's leishmanicidal effect was due to an increase of superoxide release, superoxide production was monitored using a superoxide-specific fluorescent probe (hydroethidine) in PBMC from normal donors, triggered with PMA and treated with increasing doses of DETC for 30 minutes.
ROS production was monitored using the fluorescente probe 2',7'-dichlordihydrofluorescein diacetate (H2DCFDA) (Invitrogen, Carlsbad, CA).
H2O2 production was monitored using the fluorescein-like Peroxy Green 1 (PG1) dye (Miller et al. 2007).
Similar(56)
The ROS production level was monitored using the fluorescent probe 2', 7'-dichlorodihydrofluorescein diacetate (H2DCF-DA) (Invitrogen, Carlsbad, USA).
ROS production in isolated mitochondria was monitored using dichlorofluorescin diacetate (DCFH-DA) assay [37].
Ethanol production in the culture sample was monitored using gas chromatography.
The stepwise production of ethanol and squalene was monitored using the Bioscreen.
During the last 7 d of each period, dry matter intake and milk production were recorded and lying behavior was monitored using electronic data loggers.
Production of viral and cellular proteins was monitored using western blotting and autoradioautography.
Mitochondrial membrane potential (MMP) and ROS production were measured as in Leanza et al. Briefly, MMP was monitored using tetramethylrhodamine methyl ester (TMRM; 20 nM), while ROS production was assayed using MitoSOX (1 μM).
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