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ROS production was estimated using a superoxide anion radical-specific oxidation-sensitive fluorescence dye, dihydroethidium (DHE) (Sunohara and Matsumoto [2008]) with a minor modification.
The effect of different pH values and temperatures on the bacterial growth and the enzyme production was estimated using an experimental statistical design; maximum amylase production and bacterial growth was obtained at pH 7.0 and 50 °C.
After 24 h, IFN- γ production was estimated using the Biosource IFN- γ ELISPOT-assay kit (Biosource International, Camarillo, CA, USA) under the manufacturer's protocol.
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The annual energy production is estimated using the joint distribution of wind speed and direction.
Cost of production is estimated using analytical process models dealing with imprecise design information.
Monthly wind energy production is estimated using the Weibull-representative wind data for a total of 96 months, from 5 different locations in the world.
However, the effect of granulometry and chl a concentration profile in light attenuation on irradiance and on fluorescence signal in the photic layer need to be taken into account when primary production is estimated using PAM.
When using the AP2, the SW and WP domestic production were estimated using the IRW as independent variable for the linear regression model for 50% and 46% of the countries, respectively.
Respective cellulase and xylanase productions were estimated using glucose and xylose calibration curves.
In parallel, daily above-ground biomass production (NPPshoot) was estimated using a logistic growth equation, fitted on periodic biomass samples.
Under Model 5, schooling quality production function was estimated using weighted least squares, with the number of individuals from each source country is used as a weight.
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CEO of Professional Science Editing for Scientists @ prosciediting.com