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Enteric CH4 production was determined using respiration chambers and total rumen content was sampled for the determination of fermentation characteristics and molecular biology analyses (cDNA-based length heterogeneity PCR, quantitative PCR).
Intracellular ROS production was determined using the dichlorofluorescein (DCF) assay [44].
Maximal laccase production was determined using a second-order central-composite design and analyzed by the response-surface method.
Maximum sorbitol production was determined using a second-order central composite design and analyzed by the superficial response method.
In the second experiment, where we studied oxygen succession in a carbon-enriched environment, primary production was determined using 3-h incubations at mid-day with 100 ml of water in Nunc culture bottles to which 3.2 µCi of Na14CO3 where added.
Nitrite production was determined using a standard curve made from serial dilution of sodium nitrite (range 200 3.125 µM).
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An optimal feeding strategy for lipid production is determined using the state-of-the-art interior point optimizer (IPOPT) solver.
The optimum levels of the components that positively affect enzyme production were determined using response surface methodology (RSM) based on central composite design (CCD).
The process parameters particularly nature of the carbon-limiting substrate, medium composition and nutritional supplements for L-dopa production were determined using Plackett-Burman design.
Potential differences in T- and B-lymphocyte surface marker expression and cytokine production were determined using a Mann-Whitney U test.
After peripheral blood CD14+ monocytes were stimulated with various doses of CRP, RANKL mRNA expression and protein production were determined using real-time PCR and enzyme-linked immunosorbent assay (ELISA) in the culture medium, respectively.
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