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Analysis of the determined product specific kinetics indicates that the predominant HFP production pathway at low residence times (<3 s) is via the reaction of TFE with difluorocarbenes.
Thus, after implementation of the isoprene production pathway by metabolic engineering, acetate still would be the main end product.
One AGO protein has been implicated in both rice development and the RNA production pathway.
Also, another vanillin production pathway derived from the vanillin glucoside was proposed.
Metabolic engineering of the cadaverine production pathway is also a promising approach for increasing cadaverine production.
The acetate production pathway competes with isoamyl acetate production for the common intracellular metabolite acetyl-CoA.
In order to metabolically tailor the 1,3-PD production pathway multiple strategies have been pursued.
The glycerol production pathway was impaired by deletion of the genes FPS1 and GPD2.
A key gene in the ethanol production pathway is the bifunctional aldehyde and alcohol dehydrogenase, adhE.
At the same time, this production pathway is accompanied by a comparatively less complex target preparation and separation procedure.
Thus, the vanillin production pathway with decarboxylation will not be suitable for the biotransformation in A. luchuensis.
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