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However we observed different profiles of productive viral infection between the two tissue types of decidua, and a higher production of virus was observed in DP infection compared to DB infection.
In other cases, the production of virus by the host cell may cause the death of the cell.
At the cellular level, possible outcomes of infection range from production of virus particles without damage to the cell, to cell death, or occasionally cell transformation.
The mPCR assay developed here will aid in the production of virus free planting materials and rapid indexing for certification of citrus budwood programme.
The processes that occur during the latent period include (i) translocation of viral genetic material from the periplasm into the cytoplasm (ii) replication of genetic material and production of virus particle components (iii) packaging of viral genomes into viral heads (iv) disruption of the cell surface and release of viral progeny.
However, it is not known whether RdRp is necessary for efficient production of virus particles.
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The mPCR provides a useful rapid method for detecting multiple viruses in citrus plants that will aid in the production of virus-free citrus plants for certification programs.
Management of tristeza depends on destruction of CTV infected field plants, production of virus-free planting material for new orchard establishment and controlling viruliferous aphid vectors responsible for field spread of the pathogen.
For the production of viruses in the presence of LEDGINs or DMSO, the transfection medium was replaced with fresh OptiMEM supplemented with CX14442 (1.2 μM, 20 × IC50, synthesized by the KU Leuven Centre for Drug Design and Development (CD3)) or an equal volume of DMSO.
It involves the production of virus-derived small interfering RNAs that drive degradation of viral RNAs in a sequence-specific manner.
The RNA silencing-based antiviral defence involves the production of virus-derived sRNAs (vsiRNAs) by DCL activities that drive degradation of viral RNA in a sequence-specific manner.
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