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In this study, the JSC4 strain cells were cultivated under various sea salt concentrations (0.5, 2.0, 3.5, and 5.0%) for 10 days under nitrogen-depleted conditions with regular monitoring of biomass production, nitrate concentration, lipid content, and lipid productivity.

Positive for citrate utilization, urease, and glucose while negative for lysine, ornithine, TDA, H2S production, nitrate reduction, adonitol, lactose, arabinose, and sorbitol.

Bacterial isolate was identified on the basis of colony morphology and different biochemical tests such as Gram staining, catalase test, oxidase test, citrate utilization, fermentation of carbohydrates, H2S production, nitrate reduction, indole and urease test (Benson 1994).

Biochemical tests were performed to confirm the E. coli using catalase test, Simmon's Citrate Agar, sugar fermentation on Triple Sugar Iron Agar Oxoid CM0277, Basingstoke, England), Gelatin liquefaction, Indole Production, Nitrate reduction, Urease production, Voges proskaur, Methyl red and Presumptive test.

Species level identification of the isolates was done by standard biochemical tests (niacin production, nitrate reduction, catalase and aryl sulfatase activity, Tween hydrolysis, thiopen-2-carboxylic acid hydrazide sensitivity, etc).

Polymorphisms were also detected in several biochemical characters (indol production, nitrate reduction, and methyl red and oxidase tests).

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Based on the production of nitrate reductase, 95 strains were attributed to the ovis biovar (nitrate reduction negative) and three to the equi biovar (nitrate reduction positive).

The range of HRTs tested here resulted in various undesirable pollution swapping by-products from the denitrifying bioreactors: nitrite production when nitrate removal was not complete and sulfate reduction, chemical oxygen demand production and decreased pH during overly long retention times.

Chronically elevated N deposition is theorized to induce a series of changes in ecosystem processes, including increases in foliar and soil N, decreases in soil C N ratio, increases in the net production of nitrate in soil, and increased leaching of N, largely nitrate, in streamwater.

Examining reasons for the inefficient use of N fertilizer, we hypothesized that sugarcane resembles other giant tropical grasses which inhibit the production of nitrate in soil and differ from related grain crops with a confirmed ability to use nitrate.

Abundant C availability and the acidity of soils in this forest favoured ammonium production over nitrate.

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