Sentence examples for product were subsequently from inspiring English sources

3 µl of the first amplification product were subsequently mixed with 22 µl of "inner" amplification mix, which contained inner fluorescent microsatellite primers at 0.5 µM, master mix 2 × (12.5 µl, Qiagen Multiplex PCR kit), and double-distilled water up to a 25-µl final volume.

The product was subsequently cloned into the pET-15b (Merck/Novagen) expression vector for production of protein with an N-terminal His tag.

The product is subsequently reacted with an amine to give a spiro lactam.

The PCR product was subsequently digested with Eco53KI/PacI and inserted into excised insulin promoter vector to yield the final construct.

This product was subsequently treated with formaldehyde and morpholine or diphenylamine to get the desired product.

The product was subsequently dried in a vacuum oven at 40 °C for 12 h.

The obtained PCR product was subsequently sequenced.

Each PCR product was subsequently sequenced in both directions in a total volume of 10 µl.

The WGA product was subsequently diluted 1∶16 in water and then directly used for MALDI-TOF MS genotyping.

The eluted product was subsequently cloned into mammalian expression vector pcDNA3.1 and transformed into competent Escherichia coli DH5α cells.

The PCR product was subsequently digested with SalI restriction enzyme and cloned in pET30b (Novagen, Madison, WI, USA) expression vector at SalI site.