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The integrated density of the full-length EGFP-SMN and the N-terminal cleavage product were quantified using ImageJ (http://rsbweb.nih.gov/ij/).
Bands corresponding to each specific PCR product were quantified via the densitometric scanning of the exposed film using the Bio-profile Bio-ID application.
Given that PGI2 has a short half-life (<20 minutes at physiological pH), levels of 6-keto-prostaglandin F1α (6-keto-PGF1α), the PGI2 stable degenerative product, were quantified to evaluate the PGI2 secretion from late EPCs.
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The His-Leu product was quantified fluorometrically.
For low efficiency reactions, Conversion of substrate to product was quantified as follows.
The absorbance of the product was quantified using a MTP-300 microplate reader (Corona Electric).
The amount of colored product was quantified using an ELISA reader at 406 nm.
The intensity of the product was quantified using Quantity One, Bio-Rad.
The reaction product was quantified by absorbance at 570 nm.
The RNA product was quantified by absorbance at 260 nm.
Concentration of each purified PCR product was quantified.
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