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The crystal structure and phase purity of the intermediate as well as the final product were analyzed by X-ray diffraction measurement and the crystallite dimensions evaluated by using the Scherrer's formula.
The composition, quality, and molecular structure of the product were analyzed by FTIR spectroscopy.
Additionally, the economic characteristics and fields of application for each product were analyzed.
The structure, phase and morphology of synthesized product were analyzed by the standard characterization techniques.
The effects of different temperature (microwave, or not), reaction solvents (DMF, 1,4-dioxane, THF and dichloromethane) on the yields of product were analyzed.
5 µl of each PCR product were analyzed on a 1.5% agarose gel, another 2 µl were purified with the NucleoFast 96 PCR kit (Macherey-Nagel) and used for the sequencing reaction (BigDye Terminator Kit, Applied Biosystems, Foster City, CA) that was performed according to the manufacturer's instructions.
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The purity of the final product was analyzed by FPLC.
Final product was analyzed using 1H-NMR spectroscopy in CDCl3.
The product was analyzed by ESI MS in negative mode.
The assay product was analyzed by HPLC.
PCR product was analyzed by agarose gel electrophoresis.
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