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The RT-PCR analysis was considered positive for expression only for UCEs yielding a specific PCR product validated by sequence analysis in 3 replicates.
Restriction enzyme analysis performed with EcoRI and EcoRV on the RT-LAMP product validated no nonspecific reaction in RT-LAMP assay.
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We will focus on the historical circumstances in which a specific social and commercial environment came to exert a strong influence on upper atmospheric physicists, and in which realism as a product validating the "truth" of certain practices and beliefs arose.
Only marine fisheries products validated as legal by the competent flag state or exporting state can be imported to or exported from the EU.
Fragment-length analyses of EGFR exon 19 PCR products validated this deletion in sample 27 (Supplementary Fig. S11).
The RACE-PCR products validated the previous manual re-annotations for six genes [ 7].
Sequencing of cDNA amplified from the prtA, prtB, prtE and kexB gene products validated the FGENESH predictions for these genes.
The movement to bring datasets into the scholarly record as first class research products (validated, preserved, cited, and credited) has been inching forward for some time, but now the pace is quickening.
Importantly, this method retains the ability to sequence reverse transcription polymerase chain reaction (RT PCR) products, validating microRNA-specific amplification.
After the PCR amplified product was validated as the intended product, it was purified using a PCR gel purification kit (Qiagen) according to the manufacturer's instructions.
The final product was validated by Agilent Bioanalyzer 2100 (Agilent, Santa Clara, CA).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com