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Optimizing the expression of efflux transport in biocatalyst cells can lead to increased product yield, improved product tolerance of production organisms, and maximized product recovery from the extracellular medium.
It has been reported that genetically engineered microbes, especially anaerobic strains, show efficient ethanol production and high product tolerance [ 5].
Ease of biocatalyst production, rate of enzyme activity, substrate, and product tolerance are the main factors considered for the commercialization of the enzyme based bioprocesses.
The process has seen significant progress in recent years: inhibitor sensitivity, product tolerance, ethanol yield and specific ethanol productivity have been improved in modern industrial strains to the degree that up to 20% (v/ v) of ethanol can be produced from starch-derived glucose [ 3].
The product tolerance of the NHaseM (αS122C) mutant was enhanced while its activity decreased by 30%.
Based on these relationships, the total cost of model can be expressed as a function of product tolerance from which the optimal tolerance limits can be found out.
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To secure good geometrical quality in the final product, tolerances, locator positions, clamping strategies, welding sequence, etc. are optimized during design and pre-production.
These results validate our approach in the selection of wild Saccharomyces cerevisiae strains with thermo-tolerance and degradation products tolerance properties for lignocellulosic biofuel production.
To obtain a recombinant Rhodococcus or Nocardia with not only higher enzymatic activity but also better operational stability and product-tolerance ability for bioconversion of acrylamide from acrylonitrile, an active and stable expression system of nitrile hydratase (NHase) was tried to construct as the technical platform of genetic manipulations.
To assure feasibility and economy of mechanical product design, tolerance design should be incorporated into conceptual structure design phase.
The thermal behavior of the product showed tolerance to high temperature exposure, i.e. 80-120°C 80-120°Cnchanged exothermic peaks wheredetermined in the thermograms before and after exposunchanged
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