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Incubation of Srs2 with such a DNA substrate resulted in the normal generation of unwound product (Supplementary Fig. 5).

The target sites were amplified using primers generating a 350 600 bp long PCR product (Supplementary file 1E).

The fraction was collected and lyophilized to give a yellow powder as the final product (Supplementary Figure S1).

Primers and probes were obtained from Applied Biosystems as Assays-on-Demand SNP genotyping product (Supplementary Table 1).

Primers were designed for the 5′ gene of the putative fusion product (Supplementary Table 1) and RT-PCR performed, with ACTB used as a positive control.

The Vrs1 insertion resulted in the generation of a stop codon, producing a polypeptide 14 residues shorter than the HvHox2 product (Supplementary Fig.  3).

Both libraries were screened by hybridization on colonies with probes constituted first by an Abi cox1 cDNA and by cloned PCR products (Supplementary information files Figure S1).

The complete sequence (29.902 nt, GenBank Accession number: EU314927) of the cox1 gene of the button mushroom Agaricus bisporus (Abi) was determined for a subculture (Bs 518) of the traditional brown cultivar C9 from overlapping cloned BamHI and EcoRI restriction fragments and cloned PCR products (Supplementary information file, Figure S1).

Agarose electrophoresis was performed to identify the amplified products (Supplementary Figure 1A C).

Most of the cleavage sites have been mapped by N-terminal sequencing of endogenous cleavage products (Supplementary Figure S2).

We were able to align C-terminal regions of the AtpF N and AtpH gene products (Supplementary Fig. S6).