Sentence examples for product strands from inspiring English sources

The probe must be complementary to one of the two product strands such that the extending polymerase will encounter the probe and cleave it, separating the probe's two labels and activating the probe's fluorescence through its exonuclease activity.

There is a quantitative relationship between the number of target nucleic acid strands initially present in a sample and the number of product strands synthesized at any given thermal cycle.

As discussed below, the arrangement of the tunnels in LACV L protein suggests an elegant strategy for RNA synthesis whereby the polymerase forces separation of the template and product strands and directs each down distinct exit channels on opposite sides of the molecule.

In the first step of LAMP, an inner primer starts the reaction and the newly produced strand is displaced by annealing of an outer primer on the same target strand and subsequent synthesis of complementary product strand.

The displaced product strand (primed by inner primer) itself serves as template for synthesis of new strand primed by the second inner and outer primers, which hybridize to the other end of the target DNA; the strand adopts stem-loop structure.

A leader sequence (GTTTCTT) was appended to the 5' end of each reverse primer to catalyze the non-templated enzymatic addition of a nucleotide to the 3' end of the labeled product strand [37].

The labelled PCR product strand was then isolated, and the fluorescent products hybridized to a BeadChip.

The shorter product strand labeled with Cy3 is released, and fluorescence is simultaneously recovered.

Multiple RdRPs are present on D m, and each RdRP produces one product strand and then dissociates with rate h.

The labelled PCR product strand is then isolated and the fluorescent products were hybridised to Illumina Ref-8 Expression BeadChips and scanned.

When a DNAzyme leg cleaves a substrate strand (in the presence of Zn2+), the leg is "released" from the shorter product strand and will anneal to the next available full substrate.