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Table 1 The primer sequences, melting temperature, their respective product size, and gene bank accession number Target Gene Primers Melting Tm (°C) Product size Gene bank accession no.
Reducing product size is a form of invisible price increase".
The expected product size for a fully spliced transcript is 395 bp, while the expected product size if intron 8 was retained is 1499 bp.
Transgene PCR product size: 306 bp.
It includes multiple layouts and product size and color options.
Transgene PCR product size: 351 bp; internal control PCR product size: 632 bp.
The uncut PCR product size was 344 bp.
Expected product size obtained from Gramene and observed product size for the SSR markers used in this study.
PCR product size was 354 base pairs (bp).
The sequence of primers, expected product size and references are given in Table 3.
GeneRuler™ 100 bp DNA Ladder (Fermentas, Life Sciences) was used to determine product size.
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