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At 2 °C/min heating rate, the inflection temperature Ti is an indicator that allows estimating the optimal isothermal drying temperature Td about 15 30 °C below Ti, depending on the product sensitivity to heat treatments and the kinetics of water molecule diffusion through the amorphous matrix.
A comparable performance was reported by Bristow et al 19 for the same test product (sensitivity and specificity, 99.7%).
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The calculated NGAL cut-point of 94 ng/ml that maximized the product of sensitivity times specificity achieved a sensitivity of 0.65 and a specificity of 0.65.
In the case of the aminopeptidases, the leucine aminopeptidase 2 showed a higher impact on the hydrolysis compared to the leucine aminopeptidase A, but exhibited the highest product inhibition sensitivity.
The optimal cutoff was chosen as the highest product of sensitivity and specificity.
Optimal cut-off points were defined by maximization the product of sensitivity and specificity.
At low product concentrations, sensitivity was increased by monitoring the most characteristic ions in selected ion monitoring (SIM) mode.
We found that the highest product of sensitivity and specificity was seen with gal-3 levels of 17.72 ng/mL.
The optimal cutoff value was obtained by calculating the maximum value for the product of sensitivity and specificity.
The best predictive cut-off value was defined as that which gave the highest product of sensitivity and specificity, positive predictive value (PPV) and negative predictive value (NPV).
To obtain an optimal output, we select SSPN as our fitness function that is described as the product of sensitivity (SN), specificity (SP), positive predictive value (PPV), and negative predictive value (NPV).
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