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The LIFECODES HLA-DRB1 eRES product resolves most CWD-CWD allele pairs.
The LIFECODES HLA-DQB product resolves most CWD-CWD allele pairs.
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Helicase reactions were carried out in the presence of ATP and the products resolved on non-denaturing PAGE.
Amplification was carried out over 32 cycles and the products resolved on a 1% agarose gel.
The reactions were terminated and the products resolved as described above.
After 15 min at 37 °C, EDTA and loading dye were added and products resolved on 1% agarose gels.
Labeled products were resolved by capillary electrophoresis.
An aliquot of the PCR product was resolved on a 1% agarose gel.
APE1 incised product was resolved on a 15% polyacrylamide-7 M urea gel.
Cleavage products were resolved on 2% agarose gel.
The digestion products were resolved on 21% polyacrylamide/8 M urea gel and quantified after autoradiographic visualization.
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