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The quality of amplification was controlled by the amplification efficiency as represented by the increase in product per cycle.
A 100% efficient amplification, resulting in a doubling of amplification product per cycle, was assumed (a requirement for the use of delta-delta-Ct method).
This relative fold change was determined by assuming a near perfect amplification resulting in a doubling of amplification product per cycle.
Pressure-time product per cycle (PTP) during the triggering phase tended to be higher for the simple ETV, as compared to ICU-like and sophisticated ETV (513 ± 247 versus 133 ± 119 versus 154 ± 103 cm H2O ms respectively; P = 0.08).
Triggering performance was assessed according to the following criteria: 1) triggering delay (DT) between onset of the airway pressure decay and flow delivery; 2) pressurization delay, which is the time at which the airway pressure signal rose (DP), and 3) airway pressure-time product per cycle (PTP) during the trigger phase, defined as the area under the Paw signal during the DT interval.
Similar(55)
This analysis indicated a gain of PCR products per cycle, from the empty and filled sites, respectively, of ∼1.8 and ∼1.6 over 20 cycles of PCR.
In this droplet-based embodiment, up to one million reactions are parallelized, leading to an average error of 15%, to be compared with qPCR, which produces a quantitative error of 100% in just a single cycle (as it doubles the product once per cycle) 61.
37, 53 In our study, we asked participants to report flow heaviness on the basis of the number of sanitary products used per cycle.
If the amplification is 100% efficient (percentage expressed by: E − 1), then the amount of PCR product should be doubled per cycle, resulting in an E-value of 2. Primers were considered reliable if they featured efficiency values between 92% and 108 % with a correlation coefficient, R (i.e. how well the standard curve regression line fits the data), >0.99.
The ideal power is the product between the net work per cycle and the number of revolutions per minute: {text{EP}} = {text{rpm}} cdot W_{text{net}} (18).
Pegfilgrastim is administered just once per cycle, whereas shorter acting products are used daily.
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