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PCR product intensity for WISP3/CCN6 was reduced 5.5 ± 0.6-fold (n = 4).
WISP3/CCN6 PCR product intensity did not change during adipogenic differentiation.
The product (intensity score × the percentage of immunoreactive tumour cells) yielded a final score ranging from 0 to 300.
The PCR reactions were optimized for number of cycles to ensure product intensity within the linear phase of amplification.
The product (intensity score × the percentage of immunoreactive tumour cells) yielded a final score of 0 300.
For semiquantitative PCR, reactions were optimized for the number of cycles to ensure product intensity within the linear phase of amplification.
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Separate amplifications of serial dilutions of template DNA were carried out to establish that the measured product intensities were within the linear range.
PCR product intensities on agarose gels were measured using the AlphaDigidoc AD-1200 densoftwarey softwAlphaAlpha Innotech).
In cases of differential PCR product intensities densitometry was performed using an LTF densitometer and the bioprofile software (LTF, Wasserburg, Germany) MSC undergoing osteogenic differentiation were subjected to immunohistochemical analysis for CYR61 expression.
Quantifications of PCR products intensities (reflecting the transcripts levels) were used to calculate the female antennae/male antennae (FA/MA) expression ratio for each OBP as well as for both control genes.
Significant indirect (and total) effects are seen in paths that link market orientation and expected future performance (through product innovation intensity, market innovation intensity, and satisfaction with performance).
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