Contextual analysis utilizes the information gleaned from the association of uncharacterized domains with other domains of known function and the tendency of genes whose products functionally interact to associate in conserved gene neighborhoods or predicted operons.
Collectively, these data strongly support the hypothesis that the transformed cells were hypersensitive to vorinostat-mediated apoptosis due to acute transcriptional alterations in the balance of pro-death and pro-survival BCL2 family genes, whose protein products functionally converge to govern the integrity of the mitochondrial outer membrane.
The spatial alignment between functionally important components is what makes the product function.
"There's no real reason that a brand needs to die," he told me, unless it is attached to a product that "functionally doesn't work".
Expression of this gene is under the control of two distinct promoters, and the properties of each product are functionally associated with different stages of differentiation.
For example, when the drug target is the gene product possessing functionally redundant proteins (e.g., VPH1 and STV1 [40]) and/or is associated with relatively lower enzymatic activity (e.g., Rnr3p [41], Hmg2p [27], and Fks2p [32]).
This gene product is functionally related to the actin cytoskeleton via the cAMP-dependent protein kinase A (PKA) [ 37, 38].
12, 13 The mRNA expression of Igf2bp3, whose product is functionally correlated with Igf2 mRNA and H19 mRNA, 14 was also increased in CCl4-induced tumors (Fig. 2).
We favor the former scenario for the following reasons: 1) the gnd gene product is functionally plastid-related, and is enzymatically localized to the plastid in green plants [ 17]; and 2) the overall tree topology in Fig. 1 is consistent with a recent multigene phylogeny of eukaryotes based on slowly evolving nuclear genes [ 19].
To gain insight into the predicted functional characteristics of the current dataset, translated products were functionally annotated using the Gene Ontology (GO) classification as implemented in the Blast2GO software [ 34], as well as using the InterPro classification [ 35].
