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The efficacy of each individual protein was determined at a uniform dosage with a high-throughput protein product assay followed by a cell-based functional assay without requiring protein purification.
We performed internal quality control for GSH measurements as described for the lipid fluorescence product assay.
The reaction mixture was incubated for 15 min at 30°C followed by radical-cation product assay.
In addition, we used a quantitative fibrinogen/fibrin degradation product assay for analysis of the status of intravascular fibrin formation and fibrin dissolution.
All combinations of product, assay, and solvent types were not used, sometimes because unstressed products of a resin type exhibited significant EA (i.e., 9/11 unstressed products made from acrylic resins exhibited EA).
After sufficient data are obtained using NIR to determine end point through a PQS-approved continuous process plan, it is desired to use NIR to replace finished product assay testing.
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This pilot-scale separator also produced a non-magnetic product assaying 0.041% Fe2O3 at an iron removal rate of 58.70% (nearly 96% for magnetic minerals) from a feldspar ore assaying 0.068% Fe2O3.
The significance of these developments for the analysis of biotechnological products is illustrated using three examples of product assays conducted on currently commercially available lab on a chip instrumentation.
When they are respectively optimized at 5 r/min, 5° and 8.3 L/min, it produced a non-magnetic product assaying 0.0166%Fe2O33 at an iron removal rate of 43.97% (nearly 95% for magnetic minerals) from the ore assaying 0.0359% Fe2O3; such a separation performance correlates well with the industrial operation of full-scale WBHGMS separators in purifying the ore.
Extract of P. minus was shipped to Brunswick Laboratories, Norton, MA, an independent contract laboratory specialising in standardised natural product assays, to test for ORAC values.
Although in general depletion assays are less desirable than assays that measure formation of a product, there may be cases where products are not accessible for measurement or such product assays cannot be applied due to interference.
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