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Producing viruses involves much more expensive equipment to do things like rearing host cells.
We always thaw a fresh vial of 293T cells when we are producing viruses.
Viruses with similar ancestral lineages, e.g. the HK821-like viruses from China, evolve at different rates and in different ways in domestic poultry in northern compared to southern Vietnam, producing viruses that are genetically distant both within- and between-regions by 2007.
For producing viruses containing IN.eGFP or IN.mEos3.2, the respective expression vector was co-transfected with the proviral plasmid at a molar ratio of 1 4.5.
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The viable infected cell population is further subdivided into cells producing virus, cells producing recombinant protein, and cells producing neither.
However, the number of infected cells producing virus would be diminished by the fraction that were killed before they started production.
During highly active anti-retroviral treatment (HAART), macrophages keep producing virus because tissue penetration of antiretrovirals is suboptimal and the efficacy of some is reduced.
This has been linked in part to poor stimulation of B cells producing virus-neutralizing antibodies.
6, 9 A reservoir can be classified as latent, if the infected cells are not producing virus but retain the capacity to do so.
The problems with that assumption are obvious if one considers that natural selection has produced viruses, predators, and nepotism.
Virus replication delay (tau_{2}) represents the time necessary for the newly produced viruses to become mature and then infectious (see [10, 14, 22]).
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