When the Act-GS-255B ubiquitous driver was used to drive expression of the p53-259H transgene in adult flies, it produced life span extension in females, consistent with previous results using the Elav-GS driver [ 9], and therefore demonstrating that the Act-GS-255B driver can produce increased life span when combined with an appropriate target gene.
Such stars spend most of their energy-producing life span as a main sequence star.
We show here that feeding rapamycin to adult Drosophila produces the life span extension seen in some TOR mutants.
Over-expression of a dominant p53 allele (p53-259H) in adult flies using the ubiquitous driver produced increased life span in females but not males, consistent with previous studies.
To confirm that the Act-GS-255B driver can produce increased life span, it was used to drive over-expression of the dominant p53 allele (p53-259H).
The Elav-GS line was backcrossed to the w[1118] strain for 9 generations and then re-tested by crossing to w[1118], and feeding drug to the female progeny produced a life span increase of 19.4%.
In contrast, during development, p53 over-expression acted in a dose-dependent manner to either reduce or increase the subsequent longevity of both male and female adults: high level expression during development was detrimental, whereas moderate over-expression produced increased life span.
Over-expression of p53-259H in adult flies using the ubiquitous Act-GS-255B driver produced increased median life span in females (+8%) but not males (-2.8%), and no life span increase when expressed in larvae (Table 3).
Females reproduce over their 3 4 month life span, producing 8 10 progeny.
The w[1118] control strain was crossed to the Gene-Switch system driver strain Elav-GS, and feeding the drug mifepristone to the progeny produced no change in life span in males, and an increase in female life span of 11.4%.
Decreased activity of the gut sodium dicarboxylate transporter NAC-3 or NAC-2 (high-affinity sodium-dicarboxylate cotransporters that accept a range of tricarboxylic acid-cycle intermediates with 4-5 catoms atobtainedtained using RNAi, produces an increase in life span varying from 15%to19%9% [ 30, 31].
