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The second strand was amplified using Advantage polymerase by 5′ PCR primer II A, 5′-A AGCAGTGGTATCAACGCAGAGT-3′. A directional cDNA library was constructed with a plasmid cloning kit (SuperScript™ Plasmid System, GIBCO/BRL) following the instructions of manufacturer, producing a library of about 1.0 × 106 independent colonies.
Finally, this approach uses a relatively small amount of target protein and is amenable to high-throughput application and automation, which is very important for producing a library of affinity reagents to the proteome.
A directional cDNA library was constructed with a plasmid cloning kit (SuperScript™ Plasmid System, GIBCO/BRL) following the instructions of manufacturer, producing a library of about 5.7×105 independent colonies.
A homozygous RGK1 line for this 5XUAS rsGFP-GUS reporter was chosen as the parent strain for producing a library using the pRGK335 trap vector (see below for library construction).
This study was successful in producing a library in which 85%% of the isolated compounds were Lipinski-compliant and therefore drug-like, however the library was biased towards reported compounds, and contained only a limited number of novel structures (around 1 %).
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They produce a library of property journals and run a plethora of charitable organisations.
Over the next five years, we will produce a library of 180 standards which will touch on most aspects of NHS care.
After Iraq's brutal history of hostage-taking, which has produced a library of videos showing ritual beheadings and shootings of bound and blindfolded captives, compliance with potential executioners is neither exceptional nor surprising.
He decided to produce a library of recordings of compositions thathad previously gone unrecorded, or had been incorrectly recorded, or were out of print-recordings that would be based on authentic scores & accompanied by printed facsimilies of the scores.
An early example of such RDM is illustrated with a project to produce a library of quantum-mechanically-optimised molecular coordinates derived from a computable subset of the National Cancer Institutes (NCI) collection of small molecules [7].
The method of giving away my best stuff for free was so effective that I produced a library of comprehensive guides on some of the most essential information in digital marketing.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com