If an animal is produced with genes that cause one muscle to overproduce IGF-1, that muscle grows, and if a tendon is injured, IGF-1 speeds healing.
β-Tubulin cDNA was produced with gene-specific primers for each of the isotype mRNAs and Reflectase reverse transcriptase (Active Motif).
In the framework of the European CATMA project [ 10, 11], a micro-array was produced with 24576 specific gene sequence tags (GSTs).
Modified DH10B strains have been produced with the Red recombination genes (exo and bet), and the gam gene, integrated into the E. coli chromosome under the control of the temperature sensitive λ cI857 repressor [ 7].
The resulting dendrogram for wtERα produced with a selected subset of genes (87 genes) (see Materials and Methods), resembled closely the dendrogram with all 588 genes (data not shown).
The ES-like cells the group produced with the four introduced genes seemed to have almost all the key properties of ES cells derived from embryos.
The results produced with Cuffdiff revealed 138 genes showing differential promoter usage in the total RNA, 131 in the polysomal with 47 common to both (Fig. 2a, Additional files 4 and 5).
Recently, mouse lines have been produced with targeted disruptions of various genes in the renin-angiotensin system, and studies of these animals have provided new insights into a well-studied physiological system.
P labeled probes of selected genes were produced with the High Prime System (Roche Diagnostics).
Multiple alignments of genes were produced with the ClustalW algorithm implemented in MEGA 4 [ 90, 95].
Genomic DNA structures for ZmSUN genes were produced with full-length cDNAs, ESTs, or EST contigs with B73 genomic DNA with SPIDEY, http://www.ncbi.nlm.nih.gov/spidey.nih.gov/spidey
