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Turing's approach was more general, using crib-based decryption for which he produced the functional specification of the bombe (an improvement of the Polish Bomba).
As adenosine is not produced, the functional relevance of two tsetse fly salivary proteins with putative adenosine deaminase activity, tsetse salivary gland growth factors 1 and 2 (TSGF-1 and TSGF-2) [28], remains unclear.
Finally, while supplement of both Gal and GalNAc to the cell culture media, βGal-transferases (βGal-Ts) would modify GlcNAc terminals on all types of glycans, thus preventing Large to modify GlcNAc terminals to produced the functional glycans.
As an example from a later stage of evolution, pointed out by Maynard Smith and Szathmáry (1997): There seem to be many ways in which multicellularity can arise, as evidenced by its multiple origins on Earth, but the origin of eukaryotes may have happened only once and may have required a very specific set of circumstances to have produced the functional characteristics we observe today.
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Indeed, the expression of DPM2-GFP restored the Large function to produce the functional glycans in the Lec15.2-LG cells, while expression of DPM2-GFP alone failed to do so in either the Lec15.2 or the Pro5 cells (Fig. 3B).
Thus, the recombinant agarase provides an efficient tool to produce the functional neoagar-oligosaccharides with potential applications in the cosmetic, food and pharmaceutical industries.
Several standards are required to produce the functional and semantic interoperability necessary to support the exchange of such data: a common reference information model, a common set of data elements, a common terminology, common data structures, and a common transport standard.
The periplasmic portion of Trg has previously been fused to the cytoplasmic portion of a histidine kinase (HK), EnvZ, producing the functional chimeric protein Trz [8].
This may be due to the lack of additional processing required to produce the functional siRNA, as has been suggested by others [14].
Large has been thought to modify O-mannosyl glycans to produce the functional glycans on α-DG to mediate its ligand binding.
Translation of s-Xbp1 produces the functional Xbp1 transcription factor, which participates to a transcription program in order to first re-establish ER function and cell survival.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com