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Virus-induced mortality (defined as cells killed by viruses per cell produced) is estimated to be >90% in seawater [ 50] and >80% in deep-ocean sediments [ 51].
The variable cost per unit produced is estimated at $1.20 and additional annual fixed costs that would be incurred if the Pip were manufactured are estimated at $20,800.
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Exhaustive EPG digestion was also performed and the concentrations of mono-, di- and tri-galacturonic acid (GalA) produced were estimated.
The quantity of 64Cu produced was estimated to be 29 μCi at the EOB.
Amounts of isoprene produced were estimated by comparison with a dilution of isoprene in ethyl acetate (Sigma-Aldrich).
The concentration of the glucose produced was estimated by GOD-POD method using a standard glucose curve prepared under similar conditions.
The yield coefficients Yxo (moles of O2 consumed per cmol of biomass produced) were estimated during growth on the different carbon sources, Table 1.
Cost per unit of energy produced was estimated in 2006 to be comparable to the cost of new generating capacity in the US for coal and natural gas: wind cost was estimated at $55.80 per MW·h, coal at $53.10/MW·h and natural gas at $52.50.
The total amount of research produced was estimated by each country separately and by each group using the results.
The amount of reducing sugar produced was estimated using the dinitrosalicylic acid (DNS) assay [ 38].
The amount of glucose remaining unused and the lactate produced were estimated in the HBSS using enzymatic assays.
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