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These observations indicate that factors beyond diet and environment influence the color of silk produced in captivity by forcible silking.
The fish used as breeders were also from the third generation produced in captivity at the Laboratoire régional en sciences aquatiques (LARSA, Laval University, Québec, QC, Canada).
Although these samples originate from a non-breeding facility, we cannot exclude with certainty that these hybrids have been produced in captivity [ 25], such that our inference about naturally occurring gene flow and hybridization should be considered preliminary.
Since 2002 over 30 000 individuals have been produced in captivity (McGuire Center for Lepidoptera and Biodiversity, University of Florida) and over 7000 have been released into suitable habitat within state and national parks in the South Florida mainland and northern Florida Keys (Daniels et al. 2008).
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Several breeding programmes have successfully produced offspring in captivity.
A source lake was a lake where broodstock was collected and used to produce juveniles in captivity, which were released in wild populations, usually at age 1+.
But they are not nearly as difficult to breed as pandas, which have not produced a cub in captivity in the United States since 2010.
One such example is the introduction of the Mega seed project in India which was a total failure as regards the objective of producing quality seed in captivity and supplementation of the said seed into the wild to rejuvenate the wild stock.
I had to find out what this frog really does, but supposedly it cannot produce its venom in captivity.
Though elk and red deer can produce fertile offspring in captivity, geographic isolation between the species in the wild and differences in mating behaviors indicate that reproduction between them outside a controlled environment would be unlikely.
Although coyotes and gray wolves are known to produce fertile offspring in captivity [44], hybridization appears to have occurred only very rarely across their extensive zone of overlap in North America [15] [18].
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