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To produce vector for retroviral infection, DNA encoding YFP-paxillin was cut from pEYFP-paxillin expression vector and ligated into a retroviral vector, pBabeNeo [54].
The resulting product was cloned into pET-19b to produce vector pTG17, as described for CTL0627.
This fragment was then subcloned into pGEM3zf+ (Promega) to produce vector pGEMT7Tet2.
The purified PCR product was ligated into plasmid pGEM-T (Promega) to produce vector pDFclone1.
The product was ligated into the NdeI and BamHI sites of vector pET-19b to produce vector pDG491.
The CT373 and CT374 (aaxC) genes were amplified using primers 5CT373X and 3CT374H and ligated into pBAD/HisA, as described above, to produce vector pDG558.
Similar(52)
These descriptors produce vectors that form metric spaces.
Auto-generated fingerprints typically produce vectors with large numbers of descriptors: for example, in the ChEBI compound database [16] (which currently contains cca.
While genetic modification of adenoviral vectors can produce vectors with modified tropism, incorporation of targeting peptides/proteins into the structural context of the virion can also result in destruction of ligand targeting or virion integrity.
Masked sequences were processed by an in-house PERL script to produce vector-free sequences.
Another system used seamless excision of piggyBac (PB) transposons to produce vector- and transgene-free mouse iPS cells.
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