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In order to demonstrate the potential of the presence of phaC as an indicator of a cyanobacteria's PHA accumulation capabilities, the ability to produce PHA was assessed for five cyanobacteria with a traditional in vivo PHA granule staining using an oxazine dye.
This method did not successfully generate bacterial isolates which could metabolise palm olein to produce PHA.
They produce PHA as carbon reserves, which they store in granules until they have more of the other nutrients they need to grow and reproduce.
Thus, it became possible to produce PHA at high yields on toxic substrate and also control its composition accurately (tailor-made synthesis).
Over the past years, process development and metabolic engineering approaches have been adopted to develop recombinant PHA production strains for improving the strains' ability to produce PHA, and for changing the PHA structures to obtain better thermal and mechanical properties.
A generic process to produce PHA by bacterial fermentation involves fermentation, isolation, and purification from fermentation broth.
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While the opportunistic pathogen P. aeruginosa has traditionally been considered the primary PHAs and RLs-producing microorganism, however it appears that the ability to produce PHAs and especially RLs (Abdel-Mawgoud et al. 2010) is in fact restricted to a limited number of bacterial species.
Many microorganisms produce PHAs naturally, but not all that fast or efficiently.
The aim of this study was to construct a recombinant strain of C. necator that can use lactose-containing waste material such as cheese whey, to produce PHAs.
The aim of this study was to construct a recombinant strain of D. acidovorans DSM39 using fats-containing waste such as udder, lard and tallow, to produce PHAs.
Huisman et al. ([1989]) studied the PHAs synthesis in fluorescent pseudomonads and confirmed that P.putida, P.fluorescens, P.aeruginosa, P.tetosteroni and P. oleovorans have the capacity to produce PHAs.
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