In this study, we designed and constructed a super twin T-DNA vector (pTRIDT313-g) containing two independent T-DNA cassettes―one for the selection gene Hyg and the other for the target gene Gus―to produce marker-free transgenic lines.
At 15 QTL, all three haplotype methods produce markers with χ2' at least 0.1 units higher than r2 for any single SNP.
To our knowledge, this is the first report on the application of the gene-targeted Profiling technique described by van der Linden and associates [26] to produce markers in Prx genes.
The binary construct pCCreloxPBt, which carries a chemically regulated Cre/ loxP system and a hybrid Cry1Ab/1Ac gene driven by maize phosphoenolpyruvate carboxylase (PEPC) gene promoter (P Pepc :Cry1Ab/1Ac T Nos ), was used to produce marker-free transgenic rice line L24 that specifically expresses the Cry1Ab/1Ac proteins in leaves and stem [ 34].
This study provides novel genomic resources to support population genetic and genomic studies of both kokanee and anadromous sockeye salmon, and has the potential to produce markers capable of fine-scale stock assessment.
After fabrication and UV-assisted optical inspection of each produced marker, its coordinate matrix is stored in a secured database, paired with information on the particular tag (serial number, production date etc).
Of the total 1536 assays, 1054 produced marker genotypes that were incorporated into the diploid, intrapecific, A. duranensis A-genome map.
The E. superba mitochondrial genome has been almost completely sequenced on two occasions with the aim of producing markers for exploitation and management of krill fisheries resources [43], [44].
Core UCEs are sequenced together with their more variable flanking regions, producing markers for phylogenetic reconstruction [ 5, 6].
Moreover, libraries were constructed in most cases with the aim of producing markers useful for genetic mapping, and di-nucleotide markers, being by far more frequent in the genomes and easy to isolate, were better suited for this scope.
