Sentence examples for produce flags from inspiring English sources
"They're almost incidental, and I think that's true for any of the people that produce flags in the United States".
GST was removed using PreScission protease to produce Flag-4E/4E2, which was then incubated with Glutathione Sepharose 4B bound fusion protein of GST and the C-terminal domain of TNRC6A (6A-C).
Human PUM2 cDNA and Aurora-A were subcloned into pFLAG-CMV-2 vector (Sigma) and pcDNA3.0 (Invitrogen) to produce FLAG-tagged and HA-tagged plasmids.
Since we did not detect any apoptosis-inducing properties of either the bacterially produced GST-IL-24 or the commercially available IL-24 (from mouse cells), we proceeded to produce FLAG-tagged IL-24 from supernatants of transfected Hek cells (Hek-IL-24) as has been described before (Fig. 8A) [7].
HEK293 cells were transfected with Flag- Slit2 or control empty Flag vector, to produce Flag-Slit2 and control conditioned media, respectively.
Full-length cDNA clones of zyg-11, gadr-6 / F47G4.2, and gadr-5 / Y71A12B.17 were cloned into pCMV-Tag2 vector (Stratagene) to produce FLAG-fusion constructs; cul-2 -Myc was cloned into pEGFP-N1 vector (Clontech), from which the GFP sequence was removed; and the HA- ELC-1/pEGFP-N1 construct was previously described (S tarostina et al. 2007).
This was his first collaboration with Steven Spielberg, who later co-produced Flags of Our Fathers and Letters from Iwo Jima.
When the test for reasonable agreement fails, VA ESSENCE produces flags (red or yellow alerts) to indicate a count, which is significantly above the predicted level.
The vibration test for a combined damper produced flag-shaped behavior of the damper, and their damping ratios and stiffnesses were assessed from the hysteretic curves.
Finally, co‐expression of the two plasmids produced FLAG labeled dimers and trimers when heated for 45s.
To detect HER2-specific Ab response, we produced FLAG-HER2 soluble protein by transient transfection of 293T cells and purification with anti-FLAG affinity gel (Sigma-Aldrich).
