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The inclusion of the reference leads to a seemingly superfluous reference vs. reference BLAST analysis that actually serves an important purpose it reveals portions of the reference that are unable to produce BLAST hits, due to ambiguous bases, BLAST filtering, or an absence of protein-coding sequences (in the case of protein or translated BLAST searches).
Over two-thirds of the raw reads (>71%) map to contigs that produce BLAST hits (Additional file 3).
Most of the raw reads (>71%) map to those contigs that produce BLAST hits (Additional file 3).
The high percentage of contigs in the E. lineata assembly that do not produce BLAST hits may be a function of contig size.
Ninety-one percent of the contigs that fail to produce BLAST hits are relatively short (100 500 nucleotides in length; Additional file 4).
Sequences which did not produce blast hits were queried against the same reference library at a less stringent (95%) identity using megablast [ 22].
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The kind of H-bombs that produce blasts in the megaton range certainly produce a sizeable portion of their energy from fusion reactions.
Of the 90,440 contigs in our transcriptome assembly, 40% (36,234) produced BLAST hits to sequences in NCBI's non-redundant (NR) protein database, while 60% (54,206) had no BLAST hits.
The title track, a "densely produced blast of layered vocals [and] strummed acoustic guitars", features a "circling bass riff" similar to that of "Picture Book" by The Kinks.
The majority of probes (86 to 91%) produced BLAST hits from the same genus that was used to design the probe (and typically, also from the same species, although this was not always determinable due to incomplete annotations).
Of the 16710 input sequences, 14334 produced Blast alignments with an NCBI invertebrate reference sequence library.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com