Using this equipment, the nitrogen-containing gas mixtures produced a buffer oxygen tension of 83 ± 2.5 mmHg (11 kPa, i.e. between average arterial and venous conditions), which was stable over time.
The resulting homogenate was centrifuged at 100,000× g for 20 min at 4°C to produce a lysis buffer-soluble fraction (detergent-soluble fraction).
Protein denaturation was achieved by diluting protein samples with different ratios of 10% SDS in buffer and buffer to produce a constant final protein concentration of 0.62 mg/ml in each case.
Compact and high-density 3D nanostructures produced via DNA origami are typically produced in a buffer containing the divalent cation Mg2+ at a concentration of ∼5 20 mM, ∼1 order of magnitude higher than its concentration in blood and tissue culture media (<1 mM).
50 µg protein was added to modified rehydration buffer (6M urea, 2M thiourea, 2% CHAPS, 2% IPG buffer) to produce a total volume of 125 µl.
Approximately 2.0 g of each sample was wet-ground with a mortar and pestle in 20 mM sodium acetate buffer, pH 5.0 at room temperature, then suspended in 20 mL of the same buffer to produce a uniform, readily-pipettable slurry.
The resulting supernatant was decanted and resuspended in a phosphate buffer to produce a 0.75 ml sample for Xpert testing.
The beads were then boiled for 10 min in 50 μl Laemmli sample buffer to produce a 'bound' sample.
The buffer approach is suitable because the method is based on the data point itself, however, the size of the buffer can produce a bias.
HEL was serially diluted in phosphate buffer to produce a standard curve of 40, 20, 10, 5, 2.5, 1.25, 0.6, 0.3, and 0 μg/μL.
