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Blood collected from each fish in set 2 was spiked with 1.5 pmol of the synthetic steroid ethinyl estradiol prior to processing to confirm the compatibility of the method with an internal standard.
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SAMtools (flagstat and idxstats) software was used to translate reads into BAM files for further processing and to confirm the percentage of properly paired and singleton reads and the percentage of mapped and unmapped reads (Li et al. 2009).
Expression of TRAF2 resulted in a significant increase in p100 processing to p52, confirming previous studies in which TRAF2 has been implicated in the non-canonical NF-κB pathway [ 10].
Manual processing was also performed to confirm the results.
Manual sequence processing was also performed to confirm results.
Differential expression of 12 genes associated with MHC antigen processing was analyzed by qPCR to confirm the hypothesis that antigen processing and presentation pathways elicit an adaptive immune response following immunization.
Since the TcBBM genomic sequence was used, RNA was extracted from these Arabidopsis lines to confirm proper mRNA processing.
QC after image analysis included (1) inspection of all MIP movies and single slice images to confirm proper processing (e.g. coregistration and ROI placements) and image labelling by subject identifiers (comparing all images per mouse) and (2) automated detection of potential outliers in the uptake data.
Three micro-liter of the PCR and 4.5 ul of the fragmentation product were electrophoresed to confirm the processing of the DNA.
Then, 3 µL of PCR product and 4.5 µL of fragmentation product were eletrophoresed to confirm DNA processing.
Experimental studies have been conducted to confirm the benefits of processing medicinal herbs to reduce their toxicity [ 7, 8].
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