The concept of coupled pausing, processing, and termination is analogous to termination at the 3′ end of genes with polyadenylated mRNAs, although the biochemical underpinnings are different.
snRNAs and replication-dependent histone mRNAs display clear coupling of processing and termination, but these RNAs are processed by distinct machineries.
Although much is known about the role of pre-mRNA processing in termination, our understanding of the characteristics and generality of terminator elements is limited.
Specifically, we were interested in amygdala activation as a neural signature of emotion processing after termination of the intentional effort to regulate.
CF IA furthermore contains Rna14, Pcf11 and Clp1, which are all essential for the 3′ end processing and termination reactions.
Senataxin mutations show a pathologic phenotype that appears to be based on pleiotropic transcription processing and termination defects [179,188,189].
Indeed, both have some experimental support, and neither appears sufficient to explain all 3′ end processing and termination events (Buratowski 2005; Luo et al. 2006; Richard and Manley 2009).
For example, the CTD S7P mark is important to recruit Integrator complex to snRNA genes, which regulates 3′ end processing and termination (Egloff et al., 2007).
The gal10 ∆56 allele (Greger et al. 2000; Kaplan et al. 2005) comprises a deletion in the GAL10 3′-UTR, resulting in compromised RNA processing and termination at GAL10, allowing transcription readthrough downstream into GAL7.
For others, such as snoRNA, snRNA and CUTs, transcript processing and termination depend on components of the CPF, CFI and the NRD complex (aka Nrd1-Nab3-Sen1-dependent pathway) [133,136,138].
