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One interesting question is whether these sorts of data processing shifts could encourage regulators in international regions outside the EU to push for a similarly extraterritorial scope for their local privacy laws — or face their citizens' data falling between the jurisdiction cracks via processing arrangements designed to shrink companies' legal liabilities.
Taken together, Drosha processing shifts may be a not uncommon event that has been largely ignored in the literature so far.
Some of the sequences were found to have slight shifts in their 5' and 3' ends, such as family mir-21, mir-124, mir-126, mir-183, and mir-184, which is a common phenomenon in miRNA cloning and could be attributed to processing shifts or enzymatic modifications of miRNAs such as RNA editing, 3' nucleotide additions or sequencing artifacts [37].
Some of the sequences had slight-shifts in their 5' and 3' ends, which could be attributed to processing shifts or enzymatic modifications of miRNAs such as RNA editing [ 34], 3' nucleotide additions [ 35] or sequencing artifacts.
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The news agency also reports that Microsoft -owned LinkedIn is one of several other multinational companies planning to make the same data processing shift for international users — with LinkedIn's new terms set to take effect on May 8, moving non-Europeans to contracts with the U.S.-based LinkedIn Corp.
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Thus, it appears that the 5' end polymorphism confined to a few miRNAs is a result of Drosha processing shift while the more common 3'end variations observed in most miRNAs is due to modifications introduced after the Drosha/Dicer processing.
Thus, we hypothesized that some other miRNAs where both 5' and 3' strands get selected into RISC and the 2 mature miRNA strands do not form a typical ds-miRNA with 2 nt overhang might also exhibit Drosha processing shift.
Although it appears that both strands could be selected from either of them, they are actually derived from two different ds-miRNAs derived from one pri-miRNA due to Drosha processing shift.
Thus, the processing shift might be a mechanism to fine tune gene expression and the change of dominant variants in different cell stages might add more flexibility to regulate target genes during T cell differentiation.
In fact, we identified that miR-342 might be another example of Drosha processing shift because although its both strands are selected, the two strands do not make a typical ds-miRNA that has 2 nt overhang in both termini.
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